Figure 4

Anti-Psl mAbs reduce attachment and cell-cell aggregation. (A) Anti-Psl mAbs (10 µg/ml) inhibit P. aeruginosa attachment to an abiotic surface. ***p < 0.0001, **p = 0.0025, ***p = 0.036. (B) Anti-Psl mAbs inhibit attachment to epithelial cells. (C) Epithelial cell attachment assay calculated area under the curve (AUC). (D) Aggregation in liquid culture. **p < 0.0028. (A,D) statistics were determined using Student’s t-test, n = 3 or more for all experiments. (C) The AUC was estimated using the Trapezoidal rule with group comparisons analyzed by one-way ANOVA with the Dunnett’s multiple comparison test.