Figure 2

Involvement of various light signaling components in Glc mediated regulation of hypocotyl elongation. 3d old short day grown seedlings of WT (Ler, Col-0) and light signaling mutants (a) phya, phyb, phya phyb; (b) hy5; and (c) pif1345 (pifq) were subjected to EODFR treatment in presence or absence of different concentrations of Glc for 3d and hypocotyl elongation growth was measured. The photoreceptor mutants phya, phyb and phya phyb and hy5 mutant defective in light signaling could respond to Glc-mediated hypocotyl elongation but were found resistant for further EODFR induced hypocotyl elongation at all Glc concentrations. The pifq mutant on the other hand showed complete resistance towards both Glc as well as EODFR induced hypocotyl elongation growth. These results suggest that for Glc-modulation of shade response an intact and functional light response pathway is needed. Values represent the average of two biological replicates and error bar represents SE. (Student’s T-test; P < 0.01; *control vs. treatment; **WT vs. mutant).