Figure 6
From: Towards personalised allele-specific CRISPR gene editing to treat autosomal dominant disorders
Investigation of a guide-specific approach to treat prevalent TGFBI mutations Using a guide-specific approach, 20 nucleotide guides for the 5 most prevalent TGFBI mutations (as shown in Fig. 3a) were targeted to wild-type and respective mutant sequence in a dual luciferase assay. The 5 guides cut with varying degrees of specificities and efficiencies. There was a significant difference between the wild-type and mutant sequence in all cases.
