Figure 3 | Scientific Reports

Figure 3

From: Automated quantification of protein periodic nanostructures in fluorescence nanoscopy images: abundance and regularity of neuronal spectrin membrane-associated skeleton

Figure 3

Performance of the automated detection algorithm to discern the presence of the MPS. (a) Image composed of 100 handpicked subregions (1 μm × 1μm each) of STORM images of axons and dendrites. The 50 subregions on the top half were selected for evidently showing the MPS, whereas the 50 on the bottom half were selected for the absence of MPS. (b) Image showing the maximum \({R}_{P}\) obtained for each subregion of (a) as the intensity values. (c,d) Distributions of \({R}_{P}\) obtained from subregions not showing MPS and clearly showing MPS, handpicked from STORM (c) and STED (d) images. The vertical dotted lines show the value \(\overline{{R}_{P}}+2.5\,\sigma \) for the distributions of \({R}_{P}\) of subregions without MPS. (e) Dependency of \({R}_{P}\) on signal to background ratio (SBR) obtained from the analysis of simulated images. Error bars indicate ± one standard deviation of 10 simulations. The shaded green area shows the range of SBR typical of STED and STORM images.

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