Figure 6 | Scientific Reports

Figure 6

From: Click chemistry-based tracking reveals putative cell wall-located auxin binding sites in expanding cells

Figure 6

IAA conjugate to fluorophore does not bind to cell wall. (a) Scheme of the generation of the conjugate of IAA and fluoresceinamine (FA) using N,N’-diisopropylcarbodiimide (DIC) and amide formation. (b,c) In vivo labelling of Arabidopsis seedlings with 10 μM for 1 hour (b) using fluoresceinamine alone and (c) its conjugate to IAA (IAA-FA). Scan of the trichoblast with emerging root hair. Note only week signal in both labellings (CW, Calcofluor White). (d) Quantification of the cell wall signal confirms no differences between FA and IAA-FA labelling. Scale bars = 10 μm.

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