Figure 7
Immunolocalization of endogenous IAA epitopes in resin embedded Arabidopsis root. (a) The control sections stained with Alexa Fluor 488 -conjugated secondary antibody only (upper panel). (b) Detection of endogenous IAA epitopes using anti-IAA as primary antibody and Alexa Fluor 488-conjugated secondary antibody (upper panel). Bottom panels: counterstain of the same sections with propidium iodide marking nuclei (PI; red channel) and Calcofluor White marking cell walls (CW; blue channel). (c) Close-up of the root tip cells as overlay with both counterstains. Asterisks depict nuclear localisation and arrowhead endomembrane accumulation. Note the predominant intracellular localisation. In a few instances, observed cell wall localization of IAA epitopes might be due to intercellular polar auxin transport. (d,e) Localization of IAA using anti-IAA antibody in a trichoblast. (d) Control labelling, with no detected signal and (e) the cell wall accumulation of IAA at the tip of outgrowing root hair (arrowhead). Scale bars = 20 μm (a,b), 10 μm (c–e).
