Table 1 The specificity identification on outer membrane mutants with the Phikmvvirus and LKA1gp49 recombinant protein. (L = lysis, H = Halo/opaque zone formation).

From: The O-specific polysaccharide lyase from the phage LKA1 tailspike reduces Pseudomonas virulence

 

Strains

LKA1

Other Phikmvvirus

LKA1gp49

Wild type

PAO1k

L

L

H

Wild type without pili

PAO1p

L

/

H

Flagella and pili mutants

awt fliC/wt pilA

L

L

H

Δ fliC/wt pilA

L

L

H

wt fliCpilA

L

/

H

LPS-mutants

bΔ rmd (A B+)

L

L

H

cΔ waaL (A B)

/

L

/

dΔ rmlC (A B Core-)

/

/

/

eΔ wbpL (A B)

/

L (except LKD16, LUZ2)

/

Derived from a

fΔ fliC/wt algC

LH

H

H

Wildtype without pili

Δ fliC/Δ algC

/

/

/

  1. aknock out mutants of fliC and pilA don’t display functional flagella or pili, respectively.
  2. b rmd (GDP-4-keto-6-deoxy-D-mannose reductase) knock out mutants are deficient in A-band LPS synthesis, but produce smooth B-band LPS.
  3. c waaL encodes the ligase for both the A- and B-band O-polysaccharide.
  4. d rmlC mutants display a truncated outer core lacking the 1,3- or 1,6-linked rhamnose residue and display no A- or B-band O-polysaccharide.
  5. e wbpL encodes the initial glycosyltransferase which is necessary for initiation of both A- and B-band LPS synthesis.
  6. f algC (phosphomannose mutase and phosphoglucose mutase) mutants are deficient in alginate synthesis, in the A- and B-band O-polysaccharide and display a truncated outer core.