Figure 6
From: Cleavage of poly(A)-binding protein by duck hepatitis A virus 3C protease
Amount of viral RNA in siRNA-mediated PABP knockdown cells. (a) At 12 hpi, the copy numbers of VP0 RNA were detected with a one-step rRT-PCR assay and compared with those of the control siRNA and the PBS control groups. (b) Relative PABP mRNA levels in the cells transfected with PABP siRNA or control siRNA and infected with DHAV were measured by performing the 2−ΔΔCt method with RT-PCR. *P < 0.05; **P < 0.01. (c) The PABP protein level in the same batch of cells was determined by immunoblotting. Cell lysates were detected with anti-PABP and anti-β-actin antibodies.
