Table 1 Primers used in this study.
From: Cleavage of poly(A)-binding protein by duck hepatitis A virus 3C protease
Primer | Sequence (5′-3′) |
|---|---|
pCAGGS-PABP-F | CATCATTTTGGCAAAGAATTCGCCACCATGGATTACAAGGATGACGACGATAAGATGAACTTTGATGTCATTAAAGGC |
pCAGGS-PABP-R | TTGGCAGAGGGAAAAAGATCTTTAAGCGTAGTCTGGGACGTCGTATGGGTAGGGGTTATTAACTGCCTTTT |
pCAGGS-PABP-Q367-R | TTGGCAGAGGGAAAAAGATCTTTAAGCGTAGTCTGGGACGTCGTATGGGTACTGAGCAGTCCAGCGAGGAC |
pCAGGS-PABP-G368-F | CATCATTTTGGCAAAGAATTCGCCACCATGGATTACAAGGATGACGACGATAAGGGTGCCAGACCTCATCCATT |
pET28-PABP-F | CATATGATGAACTTTGATGTCATTAAAGGC |
pET28-PABP-R | AAGCTTTTATGGGGTTATTAACTGCCTTTT |
pET32a-Flag-PABP-HA-F | GGAATTCCATATG GATTACAAGGATGACGACGATAAGATGAACTTTGATGTCATTAAAG |
pET32a-Flag-PABP-HA-R | TTGGCAGAGGGAAAAAGATCTTTAAGCGTAGTCTGGGACGTCGTATGGGTAGGGGTTATTAACTGCCTTTT |
G368N-F | TCGCTGGACTGCTCAGAATGCCAGACC |
G368N-R | GGTCTGGCATTCTGAGCAGTCCAGCGA |
Q341A-F | ATCCCAGCGACTCAGAACCGTGCT |
Q341A-R | CGGTTCTGAGTCGCTGGGATAGCT |
Q367N-F | TCGCTGGACTGCTAATGGTGCCAGACC |
Q367N-R | GGTCTGGCACCATTAGCAGTCCAGCGA |
β-actin-RTPCR-F | TACGCCAACACGGTGCTG |
β-actin-RTPCR-R | GATTCATCATACTCCTGCTTG |
PABP-RTPCR-F | AAGGCTTCGGCTTCGTTAGTT |
PABP-RTPCR-R | GATCCTGTCCTGCTTCATTTGC |
