Figure 7
Effects of biaxial loading on collagen deposition in GelMA-HAMA constructs and viability of surface-adherent chondrocytes. Immunofluorescence staining for (A–E) collagen II and (B,C,F,G) collagen I in (A–C) statically cultured and (E–G) intermittently loaded constructs after 28 days. Immunoreactive regions for collagen II appear green, while immunoreactive regions for collagen I appear red. Nuclei were counterstained with DAPI (blue). Viability of chondrocytes adherent to the outer surface of a GelMA-HAMA construct at day 14 under (D) free swelling conditions or (B) after 1 hour of dynamic biaxial mechanical loading (1 Hz, 30% compressive strain, 1.5 mm shear amplitude). Living cells appear green, while dead cells appear red (viability stainings presented in panels (D) and (H) were performed in a separate, short-term experiment).
