Figure 1 | Scientific Reports

Figure 1

From: Rapid Detection of Prunus Necrotic Ringspot Virus by Reverse Transcription-cross-priming Amplification Coupled with Nucleic Acid Test Strip Cassette

Figure 1

Primer set screening for RT-CPA assay of PNRSV. (A) Reaction curves of real-time RT-CPA. Fluorescence was monitored every 60 s, and one cycle shown here represents 1 min. (B) Target sequence and primer location of the primer set G7. Nucleotide positions are based on the PNRSV coat protein (CP)-encoding gene (Genbank No: NC-004364.1). The cross primer (5′-TGTTCCACCTTATAGTCCTGTACTGTTATAGTCCGAATGA-3′) covers the PNRSV-CPF (5′ to 3′) and PNRSV-MBR (3′ to 5′) sequences.

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