Figure 3

Sensitivity of RT-CPA-NATSC on PNRSV detection. (A) Comparison of the sensitivities of RT-CPA-NATSC (the upper pannel) and RT-PCR (the lower pannel) on the PNRSV detection. Lane1–5 represent 10−1–10−5 dilution of total RNA of the PNRSV-infected cucumber sample, respectively. (B) The detection limit of RT-CPA-NATSC on PNRSV. Lane 1–6 represent 50−1–50−5 dilution of pPNRSV-CP, respectively. Both the test line and the control line were indicated in lane 1-5.