Figure 1
From: STRT-seq-2i: dual-index 5ʹ single cell and nucleus RNA-seq on an addressable microwell array
(a) STRT-seq-2i workflow overview. (b and c) Distribution of molecule (b) and gene counts (c) for cortex data (Fig. 2). (d) Coefficient of variation (CV) as a function of mean number of molecules m expressed in cortex cells. The fitted line represents an offset Poisson, \(Lo{g}_{2}CV=Lo{g}_{2}({m}^{-0.5}+0.5)\). (e and f) Doublet rates as estimated by the ratio of species-specific molecules, per well, in mouse-human (e) and chicken-human (f) two-species experiments. (g and h) Single-cell well success rate when addressing 100 wells per unit by (g) limiting dilution or (h) FACS with 200 nL (left) or 50 nl PBS (right) predispensed. (i) Accuracy of FACS demonstrated by checkerboard pattern sort to 32 wells per unit.
