Table 1 Custom oligonucleotides used for STRT-seq-2i protocol.
From: STRT-seq-2i: dual-index 5ʹ single cell and nucleus RNA-seq on an addressable microwell array
Lysis/reverse transcription | STRT-P1-T31 | 5′Bio-AATGATACGGCGACCACCGATCG-TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT |
P1B-UMI-RNA-TSO | 5′Bio-rCrTrArCrArCrGrArCrGrCrTrCrTrTrCrCrGrArTrCrT-rNrNrNrNrNrN-rGrGrG | |
PCR 1 | DI-PCR-P1A | 5′Bio-AATGATACGGCGACCACCGA |
DI-P1A-idx[1–32]-P1B | 5′Bio-AATGATACGGCGACCACCGAGATCTACAC-XXXXX-CTACACGACGCTCTTCCGATC | |
Tagmentation | STRT-Tn5-Idx[1–96] | CAAGCAGAAGACGGCATACGA-YYYYYYYY-GCGTCAGATGTGTATAAGAGACAG |
STRT-TN5-U | 5′PHO-CTGTCTCTTATACACATCTGACGC | |
PCR 2 | P1_2nd_PCR | AATGATACGGCGACCACCGAGATC |
P2_2nd_PCR | CAAGCAGAAGACGGCATACGAGAT | |
Sequencing | DI-Read1-Seq | ATGATACGGCGACCACCGAGATCTACAC-NNNNNN-CTACACGACGCTCTTCCGATCT |
STRT-Tn5-U | 5′PHO-CTGTCTCTTATACACATCTGACGC | |
DI_idxP1A-Seq | AATGATACGGCGACCACCGAGATCTACAC |
