Figure 6 | Scientific Reports

Figure 6

From: Frequency and reactivity of antigen-specific T cells were concurrently measured through the combination of artificial antigen-presenting cell, MACS and ELISPOT

Figure 6

Reactivity detection of OVA257–264-specific CD8+ T cells by the AAPC-microplate method and traditional ELISPOT assay. After AAPC-bead sorting, the cells retained in each well were further incubated with the AAPC-beads for reactivity evaluation. (a) IFN-γ secretion in the OVA257–264-specific CD8+ T cell population detected by modified ELISPOT after 24-hr stimulation with the two-signal AAPC-beads in the AAPC-microplate. (b) IFN-γ secretion in the spleen cells detected by traditional ELISPOT assay after 24-hr stimulation with OVA257–264 peptide in a 96-well microplate.

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