Figure 2
From: The flexibility and dynamics of the tubules in the endoplasmic reticulum
The persistence length (L p ) of the endoplasmic reticulum of fixed MRC5 cells (STORM imaging of anti-KLC3, diffraction limited KLC3 antibody and EGFP-ER transfected cells) and live MRC5 cells (conventional diffraction limited fluorescence microscopy). (a) A histogram for the distribution of the persistence lengths for the differently labelled cells. The distribution of persistence lengths all follow log-normal distributions for the four differently labelled types of ER images in MRC5 cells. (b) The mean persistence length values extracted from the four differently labelled MRC5 cells agree within error (a 95% confidence interval) i.e. diffraction limited live cells, STORM, diffraction limited KLC3 antibody and EGFP-ER transfected fluorescence images.
