Figure 7 | Scientific Reports

Figure 7

From: Identification of novel factors enhancing recombinant protein production in multi-copy Komagataella phaffii based on transcriptomic analysis of overexpression effects

Figure 7

Effect of coexpression of helper genes on MLMH production by K. phaffii induced by methanol in shake flasks. (A) Cell growth profiles; (B) Total protein concentration in the supernatant; (C) SDS-PAGE of total protein in the supernatant, and “control” indicates the negative control. K. phaffii GS115/pPIC9K-MLMH expressing MLMH was coexpressed with helper genes (CPR6, FES1, STI1, and SSA1). Coexpression of Ssa1 was used as positive control. K. phaffii GS115/pPIC9K-MLMH/pPICZ were used as negative control. Error bars indicate the standard deviation of three biological replicates.

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