Figure 3

ARTC2.1 is upregulated on microglia upon stimulation with IFNβ. (a) mRNA levels of Ifnb1 from FACS sorted microglia (n = 5 individual experiments) of unstimulated or LPS/U0126 stimulated mixed glial cell cultures were determined by quantitative real-time PCR. (b) IFNβ levels in the supernatant of unstimulated, LPS stimulated or LPS/U0126 stimulated mixed glial cells were determined by ELISA. (c) Ecto-ART activity on microglia from mixed glial cells was measured by using eNAD⁺/1G4 in response to 24 h stimulation with rising concentrations of IFNβ (1–1000 U). (d) Induction of ecto-ART activity upon IFNβ stimulation was compared in BALB/c WT and ARTC2.1^−/− microglia. (e) Upregulation of ARTC2.1 expression on microglia upon IFNβ stimulation was measured using an ARTC2.1-specific mAb in comparison to isotype control. Data are representative of 2–3 independent experiments. Statistical comparison of two groups was performed by using the student’s t test (p < 0.05 = */p < 0.01 = **/p < 0.001 = ***).