Figure 5
CCND1 and CCNE1 facilitate EV71 replication. (a) The expression of CCND1 and CCNE1 were induced by EV71 infection. SK-N-SH cells were harvested after EV71 infection for the indicated times, and the expression levels of CCND1 and CCNE1 were measured by qRT-PCR. (b,c) Overexpression CCND1 and CCNE1 promote EV71 replication. SK-N-SH cells were transfected with Flag-CCND1, CCNE1-Flag and empty vector PCAGGS. 24 h after transfection, the cells were infected with EV71 for 15 h and then harvested for qRT-PCR (b) and western blot (c) assays. (d,e) Knock-down of the expression levels of CCND1 and CCNE1 attenuates EV71 replication. SK-N-SH cells were transfected with control siRNA (NC) or individual-specific siRNAs directed against CCND1 or CCNE1. Cells were infected with EV71 for 15 h at 48 h after transfection. Then, the cells were assayed for expression of EV71 genes, CCND1 and CCNE1 by qRT-PCR (d) and western blot assay (e). Data in panels (a,b and d) are representative of at least three independent experiments, with each determination performed in triplicate (mean ± SD). Asterisks denote significant differences between indicated samples (*p < 0.05, **p < 0.01, Student’s t test). The blots in c and e were cropped and the full-length blots were displayed in Supplementary Information file.
