Figure 6

Immunohistochemistry of Ebf3 and Meis2 in female VMP and male prostate. To examine the distribution of Ebf3 and Meis2 in mesenchymal subsets in VMP and ventral prostate (VP) we performed immunohistochemistry using P0 rat female and male reproductive tracts. Panels (a–d) show Ebf3 expression; in female (a and c) and male (b and d). Ebf3 showed a highly selective distribution within VMP and VP mesenchyme, and was absent from smooth muscle (SM) and the urethra (c, a and b). Panels (e–h) show Meis2 distribution; in female (e and g) and male (f and h) mesenchyme. Urethral epithelia (URE) and prostatic epithelia (E) were negative for both Ebf3 and Meis2. Scale bars (a,b,e and f) = 600 µm. Scale bars (c,d,g and h) = 300 µm.