Figure 4 | Scientific Reports

Figure 4

From: Profiling of the transcriptional response to all-trans retinoic acid in breast cancer cells reveals RARE-independent mechanisms of gene expression

Figure 4

IRF1 is an atRA-inducible transcription factor. (a) PASTAA analysis of transcription factor affinities identified disparate transcription factors associated with atRA-inducible genes in MDA-MB-231 as compared to MDA-MB-468. (b) qPCR was used to detect expression of IRF1 in MDA-MB-231 and MDA-MB-468 cells. (c) Relative expression of IRF1 following atRA and DAC treatment in MDA-MB-231 and MDA-MB-468 cells was determined by qPCR. A two-way analysis of variance was used to compare the effect of atRA treatment to the effect of DAC treatment (*p < 0.05, **p < −0.01). (d) The expression of STAT1 was measured in MDA-MB-231 following atRA treatment and compared using a paired student’s t-test.

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