Figure 5

IRF1 expression is required for CTSS expression in MDA-MB-231 cells. (a) shRNA knockdowns of IRF1 were generated in MDA-MB-231 and MDA-MB-468. Values were compared using a one-way analysis of variance with repeated measures (n = 4). (b) CTSS expression was measured by qPCR and compared between MDA-MB-231 and MDA-MB-468 cells using a paired student’s t-test (n = 4). (c) CTSS expression was measured in shRNA knockdowns following treatment with atRA and/or DAC. A two-way analysis of variance was used to determine the effect of IRF1 knockdown compared to atRA/DAC treatment (n = 4). (d) MDA-MB-231 and MDA-MB-468 cells were treated with DAC and/or TSA and CTSS expression was measured by qPCR. Values were compared with a two-way analysis of variance (n = 4). (e) β-values representing the relative methylation (Illumina HM450 arrays) of distinct CpG sites in MDA-MB-231 and MDA-MB-468 cells treated with DAC are compared within 1500 bp of the transcription start site (TSS) (n = 3, GSE103425). For all statistical comparisons, *p < 0.05, **p < 0.01, ***p < 0.001.