Figure 1
From: IL-27, but not IL-35, inhibits neuroinflammation through modulating GM-CSF expression
Construction and validation of Lenti-IL-27HA and Lenti-IL-35HA. (A,B). Graphic representation of the lentiviral constructs used to express IL-27 (A) and IL-35 (B). (C,D) Cytokines production was assessed by ELISA (IL-27, C) or WB (IL-35, D). (E–G). Affinity purified IL-27 (white bars) and IL-35 (black bars) were used to treat polarized CD4+ T cells and measure mRNA expression of IL-2 (E), IL-17 (F), gm-csf (G), and IL-10 (F) (mean ± sd). NC = no cytokines. *p < 0.05, **p < 0.001, ***p < 0.0001 (1 way Anova). Blot in panel (D) has been cropped, and full blot is presented in Supplementary Fig. 7. We show here one representative out of three independent experiments.
