Figure 2
From: IL-27, but not IL-35, inhibits neuroinflammation through modulating GM-CSF expression
IL-27, but not IL-35 gene therapy inhibits clinical EAE development. Intracisternally injected lentiviruses infected leptomeningeal (A, right panel) and ependymal (A, inset) cells releasing transgenes product into the CSF (A), and as shown by WB (dotted vertical line) are stitching of adjacent pictures (B). Mean clinical score of EAE mice injected with Lenti-IL-27HA and Lenti-IL-35HA (open dots) or Lenti-GFP (closed dots) both preventively (C,D), on the day after immunization, and therapeutically, on the day of disease onset (E,F). *p < 0.05; **p < 0.001 (EAE is evaluated as cumulative score using Mann Whitney; n = 15/group). We show here one representative out of three independent experiments (see Supplementary Figure 5 for the other two EAE experiments). Mice were sacrificed between 28–34 days post immunization. Blot in panel (B) has been cropped, and full blot is presented in Supplementary Fig. 7.
