Figure 5
Effects of Smad5 on the osteogenic differentiation of hPDLSCs. PDLSCs were transfected with control siRNA, siRNA-Smad5 for 72 h. (A and B) Real-time RT-PCR and western blot results showed that Smad5 was silenced in Si-Smad5-3 transfected cells. (C) ALP staining and Alizarin Red S staining of the three experiment groups. (D and E) Quantitation of ALP (D) and calcium (E) levels. (F-I) Real-time RT-PCR analysis of the expression levels of ALP (F), BSP (G), Runx2 (H), OSX (I) in the three experiment groups. The values represent the mean ± SD for triplicate samples from a representative experiment (*p < 0.05, **P < 0.01 compared with untransfected hPDLSCs.). (J) Western blot analysis indicating the expression levels of Runx2 in different experiment groups. Runx2 expression was decreased in Smad5-knockdown hPDLSCs (rightmost, Si-Smad5). (K) A schematic illustration of the hypothetical regulatory pathway through which miR-21 modulates hPDLSC osteogenic differentiation via Smad5.
