Figure 1 | Scientific Reports

Figure 1

From: Effects of neuroactive agents on axonal growth and pathfinding of retinal ganglion cells generated from human stem cells

Figure 1The alternative text for this image may have been generated using AI.

Identification of hESC-derived RGC markers. (a) Expression of representative RGC markers, ATHO7, BRN3B, TUJ1, γ-synuclein (SNCG), and ISLET1 are shown. Real-time PCR analysis was performed on total mRNA extracted from hESC-derived RGCs on D30. BRN3B is expressed at high levels (>1,000×) at D30, as compared with that analysed on D0. Each mRNA expression level was first normalised to HPRT1 expression and then compared to mRNA expression on D0. (b) Immunohistochemical detection of BRN3B, ATOH7, TUJ1, SNCG, and ISLET1 and double-staining of ATOH7 and TUJ1 on D30. Whole-mount staining was performed for TUJ1; serial section staining was performed for the other targets. BRN3B and SNCG staining is mostly visible at the peripheral margins of the attached OV, while ATOH7 and ISLET1 staining is also visible at the centre of the OV. ATOH7 stains the nucleus of the RGCs. Axons stained by TUJ1 emerges from ATOH7-positive cells. Scale bars, 100 μm. Scale bar for double-staining, 15 μm. Error bars, standard deviation.

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