Figure 3 | Scientific Reports

Figure 3

From: Effects of neuroactive agents on axonal growth and pathfinding of retinal ganglion cells generated from human stem cells

Figure 3The alternative text for this image may have been generated using AI.

Effect of SEMA3A supplementation on axonal growth of hESC- and hiPSC-derived RGCs. (a) Axonal growth of hESC- and hiPSC-derived RGCs is observed by immunostaining of NFL. Axonal growth of hESC- and hiPSC-derived RGCs is starkly inhibited by 100 and 200 ng/ml SEMA3A supplementation from D27−30 (centre and right panels, respectively). The assessment from D27 is performed under RMM supplemented with 1.0% FBS and 100 ng/ml BDNF. (b) Real-time PCR analysis of mRNA expression of axonal markers, TAU, NFL, and TUJ1. In both hESC- and hiPSC-derived RGCs, the expression of, TAU, NFL, and TUJ1 appears to decrease in a dose-dependent manner. Expression levels of all markers are significantly decreased at 200 ng/ml SEMA3A supplementation (ANOVA, Tukey-HSD test, p ≤ 0.05). Scale bar, 100 μm. Error bars indicate±SD. Each column shows an average value for the studied samples. The sample size for all mRNA data is five (n = 5). NS, not significant.

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