Figure 3

Immunohistochemical localization of Oatp2a1 in normal gut and intestinal polyps. Paraffin section (4 μm) of mouse small intestine (a,d), colon (b,e), and intestinal polyps (c,f) of Slco2a1 ^+/+/Apc ^∆716/+ (+/+, a–c) and Slco2a1 ^−/−/Apc ^Δ716/+ (−/−, d–f) was incubated with anti-Oatp2a1 antibody and stained brown by immunoenzymatic reaction with DAB. Nuclei were counter-stained blue with haematoxylin. Red arrowhead and arrow indicate endothelial and stromal cells, respectively. Co-localization of CD34 (g, green) or F4/80 (h, green) with Oatp2a1 (red) was detected in polyps of small intestines of Slco2a1 ^+/+/Apc ^∆716/+. Nuclei were counterstained with Hoechst33342 (blue). Indicated white arrowheads show typical positive fluorescence for each reaction. Experiments were repeated at least three times and representative images are shown.