Figure 7
From: A novel role for OATP2A1/SLCO2A1 in a murine model of colon cancer
Effect of exogenous PGE2 on Migratory Capability of HUVECs. (a) Migratory activity of HUVECs pre-treated with indomethacin (100 μM) for 16 hrs. Migration distance of HUVECs was determined in EGM-2 containing PGE2 (3 nM) and indomethacin (100 μM) in the absence (Control, open circles) or the presence of BSP (25 μM, closed circles). Each point represents the mean ± SEM. (n = 4). (b) Extracellular PGE2 concentration was monitored for 10 hrs under the same condition. (c) Cellular uptake of [3H]PGE2 (10 nM) by HUVECs. HUVECs were pre-incubated with EGM-2 containing indomethacin (100 μM) for 16 hrs before experiment. Uptake was measured in the absence (Control, open circles) or presence of BSP (25 µM) (closed circles) at 37 °C and pH 7.4. Each point represents the mean ± SEM. (n = 3). (d) mRNA expression of cell adhesion-related genes (e.g. VE-cadherin, integrin αV and integrin β3) was evaluated by quantitative RT-PCR. HUVECs were pre-incubated with EGM-2 containing indomethacin (100 μM) for 16 hrs before experiment. Then, HUVECs were incubated with EGM-2 containing indomethacin in the absence (Control) or presence of BSP (25 μM, closed columns) for 10 hrs. Each bar represents the mean ± SEM. (n = 3). * indicates the significant different expression from Control by Students t-test (p < 0.05).
