Figure 1

Pre-pubertal mice show increased resistance to mortality from endotoxemia. (A) Pre-pubertal (Pre-P) and post-pubertal (Post-P) C57Bl/6 mice received 23 μg/mg LPS via intra-peritoneal injection. Mice were monitored twice daily and moribund individuals humanely euthanised (N ≥ 56/group). (B) Endotoxin concentrations in serum samples collected at 2- and 20-hour time-points were quantified using LAL (N ≥ 21/group). (C) Mouse weight was recorded prior to LPS injection and then at 2- and 20-hour time points. Data is shown as the per cent change in weight in comparison to time 0 (N ≥ 20/group). Per cent survival was compared using a log rank Mantel Cox test, while endotoxin concentration and weight loss were compared using Two-way ANOVA followed by Tukey’s multiple comparisons test. Significant differences between pre- and post-pubertal mice are labelled with ****(p < 0.0001).