Figure 3

Behavioural phenotypes of wild-type (WT) and Crmp4-KO mice. (a) Open-field test for 20 min with total distance moved (1), mean velocity (2) and total time spent in the central zone (3). Male WT, n = 17; male Crmp4-KO, n = 15; female WT, n = 12; and female Crmp4-KO, n = 14. (b) Elevated plus maze (distance from floor = 60 cm) comprising two open arms (27.5 × 4.5 cm) and two closed arms made of clear Plexiglas (27.5 × 4.5 × 14 cm) extending from a central (4.5 × 4.5 cm) platform for 10 min. The time spent in open (1) and closed (2) arms and the number of entries to open (3) and closed (4) arms in the elevated plus maze test. Male WT, n = 11; male Crmp4-KO, n = 10; female WT, n = 9; and female Crmp4-KO, n = 12. (c) Novel object recognition test to assess memory acquisition and retention performance. Total duration of exploring the objects during familiarisation (1) and test (2) phases of the novel object recognition test. Preference index, which expresses the ratio of the amount of time spent exploring novel object during familiarisation (3) and test (4) phases. Male WT, n = 13; male Crmp4-KO, n = 17; female WT, n = 13; and female Crmp4-KO, n = 13. Values are expressed as mean ± SEM.