Table 1 Gene expressions with significant differences among groups in adults.

From: Human CRMP4 mutation and disrupted Crmp4 expression in mice are associated with ASD characteristics and sexual dimorphism

Brain region

Genes

F-statistics, degree of freedom, and p-values for two-way ANOVA

 

Expression levels

 

For each factor and interaction

Fold differences

Male

Female

Genotype

Sex

Genotype × Sex

WT vs. Crmp4-KO (Crmp4-KO/WT)

Male vs. Female (Female/Male)

WT

Crmp4-KO

WT

Crmp4-KO

Olfactory bulb

GluR2

F(3,12) = 3.013, p = 0.072

F(1,12) = 5.843, p = 0.032

F(1,12) = 1.215, p = 0.292

F(1,12) = 1.981, p = 0.185

1.969

1.352

0.532 ± 0.188

0.564 ± 0.141

0.472 ± 0.122

0.952 ± 0.120*

VGluT1

F(3,12) = 16.871, p < 0.001

F(1,12) = 36.167, p < 0.001

F(1,12) = 11.327, p = 0.006

F(1,12) = 3.117, p = 0.103

1.389

0.833

0.815 ± 0.025†

1.009 ± 0.062*

0.585 ± 0.028

0.935 ± 0.053*

VGluT2

F(3,12) = 3.191, p = 0.063

F(1,12) = 1.300, p = 0.276

F(1,12) = 5.707, p = 0.034

F(1,12) = 2.566, p = 0.135

1.116

1.266

0.849 ± 0.051

0.803 ± 0.029†

0.920 ± 0.170

1.172 ± 0.032

GABAAα1

F(3,12) = 62.224, p < 0.001

F(1,12) = 78.549, p < 0.001

F(1,12) = 45.705, p < 0.001

F(1,12) = 62.418, p < 0.001

1.642

1.455

1.367 ± 0.120

1.459 ± 0.059†

1.259 ± 0.051

2.854 ± 0.126*

GABAAγ2

F(3,12) = 11.147, p = 0.001

F(1,12) = 20.106, p = 0.001

F(1,12) = 1.439, p = 0.253

F(1,12) = 11.894, p = 0.005

1.524

1.118

1.274 ± 0.112

1.410 ± 0.194†

0.978 ± 0.059

2.021 ± 0.126*

GABABR1

F(3,12) = 7.722, p = 0.044

F(1,12) = 16.841, p = 0.001

F(1,12) = 4.138, p = 0.065

F(1,12) = 2.184, p = 0.165

1.839

1.342

0.182 ± 0.010

0.285 ± 0.068†

0.204 ± 0.036

0.424 ± 0.012*

VGAT

F(3,12) = 7.897, p = 0.004

F(1,12) = 12.939, p = 0.004

F(1,12) = 1.182, p = 0.298

F(1,12) = 9.570, p = 0.009

1.426

1.113

0.317 ± 0.029

0.335 ± 0.057†

0.249 ± 0.015

0.476 ± 0.018*

Ncam1

F(3,12) = 7.576, p = 0.004

F(1,12) = 20.543, p = 0.001

F(1,12) = 2.124, p = 0.166

F(1,12) = 0.012, p = 0.913

1.839

1.342

1.214 ± 0.043

1.573 ± 0.051*

1.085 ± 0.096

1.462 ± 0.113*

N-cadherin

F(3,12) = 2.283, p = 0.131

F(1,12) = 6.782, p = 0.023

F(1,12) = 0.007, p = 0.936

F(1,12) = 0.061, p = 0.809

1.321

0.913

1.178 ± 0.067

1.385 ± 0.041

1.149 ± 0.153

1.400 ± 0.035

Hippocampus

GluR1

F(3,12) = 3.793, p = 0.040

F(1,12) = 1.031, p = 0.330

F(1,12) = 0.916, p = 0.357

F(1,12) = 9.431, p = 0.010

1.171

0.861

0.544 ± 0.066

0.999 ± 0.121*†

0.860 ± 0.164

0.550 ± 0.114

GluR2

F(3,12) = 2.857, p = 0.086

F(1,12) = 6.301, p = 0.029

F(1,12) = 0.106, p = 0.750

F(1,12) = 2.874, p = 0.118

1.505

1.055

0.227 ± 0.061

0.469 ± 0.044*

0.343 ± 0.022

0.400 ± 0.101

VGluT1

F(3,12) = 1.942, p = 0.177

F(1,12) = 5.266, p = 0.041

F(1,12) = 0.169, p = 0.666

F(1,12) = 0.365, p = 0.557

1.160

0.973

0.916 ± 0.079

1.017 ± 0.042

0.850 ± 0.073

1.031 ± 0.047

GABAAα1

F(3,12) = 3.623, p = 0.045

F(1,12) = 3.983, p = 0.069

F(1,12) = 5.771, p = 0.033

F(1,12) = 1.116, p = 0.312

1.211

1.265

0.194 ± 0.010

0.215 ± 0.010†

0.224 ± 0.010

0.291 ± 0.041

GABABR1

F(3,12) = 3.548, p = 0.048

F(1,12) = 0.379, p = 0.549

F(1,12) = 10.185, p = 0.008

F(1,12) = 0.078, p = 0.784

1.071

1.514

0.135 ± 0.008

0.144 ± 0.008†

0.204 ± 0.034

0.220 ± 0.027

N-cadherin

F(3,12) = 2.266, p = 0.133

F(1,12) = 5.333, p = 0.040

F(1,12) = 1.074, p = 0.321

F(1,12) = 0.392, p = 0.543

0.948

1.126

1.028 ± 0.046

0.939 ± 0.037

1.118 ± 0.032

1.096 ± 0.083

Cortex

VGluT2

F(3,12) = 3.920, p = 0.037

F(1,12) = 5.273, p = 0.040

F(1,12) = 6.351, p = 0.027

F(1,12) = 0.135, p = 0.719

0.735

0.712

0.136 ± 0.021

0.100 ± 0.009

0.097 ± 0.012

0.071 ± 0.007

GABAAγ2

F(3,12) = 5.557, p = 0.013

F(1,12) = 14.229, p = 0.003

F(1,12) = 0.018, p = 0.896

F(1,12) = 2.425, p = 0.145

0.667

1.011

0.097 ± 0.013

0.077 ± 0.006

0.113 ± 0.011

0.064 ± 0.004*

GABABR1

F(3,12) = 5.623, p = 0.012

F(1,12) = 7.181, p = 0.020

F(1,12) = 7.752, p = 0.017

F(1,12) = 1.937, p = 0.189

0.773

0.765

0.115 ± 0.012†

0.081 ± 0.008*

0.080 ± 0.006

0.069 ± 0.007

Ncam1

F(3,12) = 9.911, p = 0.001

F(1,12) = 29.650, p < 0.001

F(1,12) = 0.011, p = 0.919

F(1,12) = 0.071, p = 0.795

0.671

1.008

1.227 ± 0.085

0.839 ± 0.068*

1.255 ± 0.084

0.827 ± 0.060*

N-cadherin

F(3,12) = 20.827, p < 0.001

F(1,12) = 6.446, p = 0.026

F(1,12) = 53.076, p < 0.001

F(1,12) = 2.960, p = 0.111

0.484

1.276

1.105 ± 0.121

0.592 ± 0.029*

1.498 ± 0.117

0.667 ± 0.070*

  1. Asterisks indicate significant differences between WT and Crmp4-KO mice of same sex (*p < 0.05, two-way ANOVA followed by Fisher’s PLSD post-hoc tests). Dagger indicates significant difference between male and female mice of the same genotype († p < 0.05, two-way ANOVA followed by Fisher’s PLSD post-hoc tests). Data are expressed as mean ± SEM.
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