Figure 3
From: Elucidating the in vivo interactome of HIV-1 RNA by hybridization capture and mass spectrometry
(a) Diagram of the HIV-1 reporter virus used for functional evaluation of HIV RNA interactors. The RNA sequences coding for the fluorescent reporter proteins, CFP and mCherry, are postioned so that three CFP and one mCherry molecules are expressed following translation from the full length and the completely spliced transcripts, respectively. (b) Quantitative analysis of fluorescence from reporter proteins following target protein siRNA knockdown and 48-hours post HIV infection. (c) Western blot analysis of siRNA knocked-down target proteins. Demonstrates >90% knock down of each protein. Images and quantitative analysis of complete blots are in SI Figure S3.
