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Figure 1

From: Alternative substrate-bound conformation of bacterial solute-binding protein involved in the import of mammalian host glycosaminoglycans

Figure 1

Gram-negative Streptobacillus GAG import system. (A) A model for GAG (chondroitin sulfate C) import. Polysaccharide GAGs are depolymerized to unsaturated disaccharides by extracellular or cell-surface polysaccharide lyases. Unsaturated GAG disaccharides are incorporated in the cytoplasm by the ABC transporter (Smon0121-Smon0122/Smon0120-Smon0120) through the periplasmic solute-binding protein (Smon0123). Incorporated disaccharides are degraded to monosaccharides by cytoplasmic UGL and are metabolized by some enzymes. (B) Structural formulas of unsaturated chondroitin disaccharides. (C) The degradation of chondroitin sulfate A. Upper, before adding acetic acid; and lower, after adding acetic acid. Left, S. moniliformis; middle, Pedobacter heparinus as the positive control; and right, Escherichia coli as the negative control. (D) Decrease in intensity of Smon0123 by adding increasing CΔ4S6S was plotted after a modification based on the volume change in the cuvette.

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