Figure 6 | Scientific Reports

Figure 6

From: Alternative substrate-bound conformation of bacterial solute-binding protein involved in the import of mammalian host glycosaminoglycans

Figure 6

Interaction between Smon0123 and Smon0121-Smon-0122/Smon0120-Smon0120. Structure modeling of the complex of CΔ6S-bound Smon0123 (A) or CΔ4S6S-bound Smon0123 (B) and Smon0121-Smon0122/Smon0120-Smon0120. Dark blue, Smon0121; light green, Smon0122; cyan and light orange, Smon0120; blue, Smon0123/CΔ6S; and pink, Smon0123/CΔ4S6S. The olive ball shows calcium ion. (B) The superimposition of CΔ6S-bound Smon0123 (C) or CΔ4S6S-bound Smon0123 (D) on AlgQ2 (gray). (E) ATPase activity of the Smon0121-Smon0122/Smon0120-Smon0120 in liposomes in the absence of disaccharide (PLS, proteoliposome) or presence of CΔ0S, CΔ4S, CΔ6S, CΔ4S6S, and cellobiose. Each data represents the average of triplicate individual experiments (means ± standard errors of the means). Student’s t-test, P > 0.05.

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