Figure 5 | Scientific Reports

Figure 5

From: Access to a main alphaherpesvirus receptor, located basolaterally in the respiratory epithelium, is masked by intercellular junctions

Figure 5

EHV1 binds up to 10-fold better at basolateral surfaces than at apical surfaces of EREC with intact intercellular bridges. (a) Cells were pre-incubated with PBS or EGTA and inoculated at 4 °C for 1 h with Dio-labelled EHV1 particles (MOI 10). The percentage of cells with bound virus particles was calculated based on 5 random fields of 300 cells (left panel). The total number of particles per EHV1-positive cell was counted (right panel). Three independent experiments were performed and data are represented as means + SD, different letters indicate significant (P < 0.05) differences. (b) Representative confocal images of EREC with bound EHV1 particles (green), cell nuclei are stained in blue, the scale bar represents 10 µm.

Back to article page