Figure 1 | Scientific Reports

Figure 1

From: Adrenergic receptor agonists induce the differentiation of pluripotent stem cell-derived hepatoblasts into hepatocyte-like cells

Figure 1

Methoxamine treatment induces hiPSC-derived hepatoblasts into ALBUMIN+ cells. (A) A schematic drawing of the screening procedure. (B) The chemical structure of methoxamine hydrochloride. (C) Dose-dependent induction of ALBUMIN+ cells by methoxamine treatment. P < 0.05 (One-way analysis of variance (ANOVA) with Bonferroni’s Multiple Comparison Test). (D, E) Time course analyses of effects of methoxamine treatment on the induction rate of ALBUMIN+ cells (D) and the ALBUMIN expression (E). P < 0.05 vs DMSO and *P < 0.05 vs GFs (One-way analysis of variance (ANOVA) with Bonferroni’s Multiple Comparison Test). (F) Hepatic differentiation of multiple hiPSC lines using methoxamine treatment or GFs as analyzed by the induction rate of ALBUMIN+ cells on day 20 (Stage 3, day 8). The data are presented as mean ± SE from three independent experiments (n = 3) in (C,F). NaB: sodium butyrate, KO-DMEM: knockout-DMEM, KSR: knockout serum replacement, HCM: hepatocyte culture medium, HGF: hepatocyte growth factor, OsM: oncostatin M, GFs: HGF+OsM.

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