Table 1 Summary of qRT-PCR confirmation in cassava.

From: MicroRNA-like RNAs from the same miRNA precursors play a role in cassava chilling responses

Assay

Selected miRNA number in validation experiment (24)

Type

confirmed

not confirmed

Canonical miRNA

5/10 (50%)*

5/10 (50%)

#40, #54, miR394abc, miR168.1, miR390b.1

#28, #44ab, #60.1, miR169b.1, miR171hi

Phase miRNA

3/6 (50%)

3/6 (50%)

miR394c.2, #28.2, #40.2

miR169b.2, miR394b.2, #60.2

7/8 (83.3%)

1/8 (16.7%)

Half-phase miRNA

miR166b.2, miR168.2, miR168.3, miR171d.2, miR171d.3, miR390b.2, #54.2

#44a.2

Total

15/24 (62.5%)

9/24 (37.5%)

  1. *The numerator and denominator represent the detected miRNA number and total assayed miRNA number. The percentage of detection ratio by qRT-PCR shown in bracket.