Figure 1

Induction of splenic monocyte-derived antigen presenting cells during LPS-induced endotoxemia depends on type I IFN. Wild-type (WT), Ifnar1-KO, and Caspase-1/11-KO mice were injected intravenously (IV) with a non-lethal dose of ultrapure LPS from E. coli O111:B4 (25 μg/mouse), or PBS. Spleen cells were analyzed by flow cytometry in kinetic in WT mice (A) or 24 h after LPS injection in Wild-type (WT), Ifnar1-KO, and Caspase-1/11-KO mice (B and C). Splenic Mo-APC were gated as Lin⁻ CD135⁻ CD11b⁺ CD64⁺ to quantified their number (A) and their expression of Ly6C and MHCII has been assessed (B). Data are representative of at least two independent experiments done in triplicates. Bars indicate mean ± SEM. Statistical significance was assessed by non-parametric Mann-Whitney test. P < 0.01 (**), P < 0.001 (***) and P < 0.0001 (****) were considered statistically significant.