Figure 6

IFNα and IFNβ favour macrophage and DC progenitor development toward monocyte-derived antigen presenting cells. CD45.2⁺ MDP (gated as described in Fig. 3) sorted by flow cytometry were co-cultured with CD45.1⁺ bone marrow filler cells at day 0 in Flt3-L-dependent dendritic cells cultures. Cultures were supplemented with LPS (100 ng/ml), IFNα (100 ng/ml) or IFNβ (10 ng/ml) at day 0 and the DC and Mo-APC composition after 7 days was measured by flow cytometry (A). The frequencies of each population among the CD45.2⁺ MHCII⁺ cells were calculated (B). Data are representative of at least 2 independent experiments done in quadruplicate. Bars indicate mean ± SEM. Statistical significance was assessed by one-way ANOVA/Bonferroni posttest. P < 0.05 (*), P < 0.01 (**), P < 0.001 (***) and P < 0.0001 (****) were considered statistically significant.