Figure 3
From: Conservation of folding and association within a family of spidroin N-terminal domains
Equilibrium denaturation of NTD monomers. (a) Normalized melting curves of NTDs recorded at pH 7.0 abbreviation for ionic strength: I adjusted to 200 mM using far-UV CD spectroscopy at 222 nm (Ea: red, Lh: blue, Lg: orange, Nc: green). Black lines are data fits using the thermodynamic model for a two-state equilibrium. (b–e) Equilibrium chemical denaturation. Data were recorded by manual titration using urea as denaturant in pH 7.0 buffered solution with I adjusted to 200 mM ((b) Lg, orange circles; (c) Ea, red diamonds; (d) Lh, blue squares; (e) Nc, green triangles). Denaturation was measured using far-UV CD spectroscopy at 222 nm (closed symbols) and Trp fluorescence spectroscopy at 330 nm (open symbols). Solid lines are data fits using the thermodynamic model for a two-state equilibrium.
