Figure 2
From: Immune tuning scaffold for the local induction of a pro-regenerative environment
Infiltrating cells after 1 day from implantation in CSCL scaffold. (A) Representative SEM images showing the CSCL scaffold’ surface completely covered by cells in two different magnifications (scale bars: 200 μm and 100 μm). (B) Total number of cells recovered by CSCL and CL counted by flow cytometry. Graph represents mean values ± SD (n = 3). (C) SEM magnification to evaluate the infiltrating cells morphology on CSCL (scale bar: 10 μm). (D) Masson’s stained section revealed a massive infiltration of cells through the entire CSCL’ thickness coming from the surrounding vasculature (inset). (Scale bar: 200 μm). (E) Magnification Masson’s stained section (on the left) and a SEM image (on the right) highlighted a high level of fibronectin on the CSCL surface (yellow arrows) (scale bars: 40 μm and 15 μm, respectively). (F) Evaluation of fibronectin level of expression was performed on protein extracts from CL and CSCL scaffolds. Densitometric analysis, y-axis shows the optical density of protein expression (A) normalized against the control (B, GAPDH). Results are shown as means of three replicates ± SD. ** p ≤ 0.001.
