Figure 4
From: Immune tuning scaffold for the local induction of a pro-regenerative environment
Switching off of inflammation. (A) Representative images of Masson’s stained sections of CSCL (dotted yellow lines mark the interface with the native tissue) at 3 (top) and 7 days (bottom) (Scale bars: 100 μm, 50 μm). Images highlight dampen of the inflammation representing in a decrease of infiltrating cells from the surrounding tissue and an augment of extracellular matrix deposition. (B) Reduction in the number of cells harvested from explanted CSCL at 3 and 7 days. Values are mean ± SD (n = 3) (n = 3, **p < 0.01). (C) Representative immunofluorescence consecutive sections showing anti (IL-10)- and pro(iNOS)- inflammatory markers. Cells are counterstained with anti-Macrophages antibody. A progressive reduction of IL-10+ and iNos+ macrophages between 3 and 7 days is shown (scale bars 50 μm). (D) Flow cytometric analysis shows the percentage of IL10+/CD206+ macrophages at 3 and 7 days from CSCL implant.
