Table 1 Fluorescence markers.
From: Deep tissue analysis of distal aqueous drainage structures and contractile features
Fluorpohore | Acronym | Represents | Marker for: |
|---|---|---|---|
Endogenous | GFP | Green fluorescence protein | Cytoplasm |
| Â | Prox1-GFP | Prospero homeobox protein 1 | Endothelium of distal tract or lymphatics |
| Â | mTomato | membrane-localized tandem dimeric red fluorescent protein (td-Tomato) | Plasma membrane |
| Â | VE-cadherin-td-Tomato | Vascular endothelium cadherin | General endothelial marker |
Epifluorescence | CD31/PECAM1 | Cluster of differentiation 31/Platelet endothelial cell adhesion molecule | General endothelial marker |
| Â | F-actin | Filamentous actin | Contractile cytoskeleton |
| Â | Hoechst 33342 | Hoechst 33342 | Nucleic acid stain |
| Â | LYVE-1 | Lymphatic vessel endothelial hyaluronic acid receptor 1 | Lymphatic endothelium |
| Â | siGLO | Fluoroscein amidite-labeled RISC independent transfection control | Nucleus |
| Â | VEGFR2 | Vascular endothelial growth factor receptor 2 precursor | General endothelial marker |
Autofluorescence | AF | Autofluorescence of elastin & fibrillar collagens (e.g. types I and III) | Elastin and fibrillar collagen |
