Figure 2 | Scientific Reports

Figure 2

From: The potential therapeutic effect of melatonin on human ovarian cancer by inhibition of invasion and migration of cancer stem cells

Figure 2

Melatonin effects on proliferation and stemness properties of cancer stem cells (CSCs). (a) Inhibitory effects of melatonin on viability of SKOV3 cells. (b) The effects of melatonin (IC50 value = 3.4 mM) on viability of CSCs and SKOV3 cells were determined after 48 h incubation. (c) The protein expression of Ki67 proliferation marker was determined after incubation of the cells with melatonin (3.4 mM) for 48 h in both CSCs and SKOV3 cells by applying FITC conjugated anti-Ki67 antibody and analyzed by flow cytometry. (d) Nanog mRNA expression in CSCs was carried out by real-time RT-PCR and compared with that expression in SKOV3 cells after incubation of the cells with melatonin (3.4 mM) for 48 h. (e) The role of melatonin in SOX2 stemness marker expression was evaluated after incubation of the cells with melatonin (3.4 mM) for 48 h using real-time RT PCR analysis Results are presented as mean ± SD of three independent experiments. Statistically significant differences are indicated as *p < 0.05 and **p < 0.01.

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