Figure 2 | Scientific Reports

Figure 2

From: Enhanced CD25+Foxp3+ regulatory T cell development by amodiaquine through activation of nuclear receptor 4A

Figure 2

Enhanced IL-2 production by AQ treatment in iTreg cells. CD4+ T cells were stimulated and induced to differentiate into effector and iTreg cells. (A) Cell supernatants were harvested at day 2 for measuring IL-2 by ELISA. *p < 0.05 and ***p < 0.0005. (B) Total RNA was harvested at day 2 and subjected to quantitative real time-PCR analysis of IL-2 transcripts. **p < 0.005. Four independent experiments were performed and similar results were obtained in each. Data shown in A and B represent the mean ± SD of four independent experiments. (C) Differentiated iTreg cells were restimulated with PMA and ionomycin for 6 h and stained with PE-conjugated IL-2 followed by flow cytometry analysis. (D) Foxp3 expression was analyzed in IL-2-positive and –negative cells of veh- or AQ-treated iTreg cells. A representative image of three independent experiments is shown in C and D.

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