Figure 5 | Scientific Reports

Figure 5

From: Enhanced CD25+Foxp3+ regulatory T cell development by amodiaquine through activation of nuclear receptor 4A

Figure 5

IL-2/IL-2R-independent CD25 induction by AQ. CD4+ T cells were induced to differentiate into iTreg cells and incubated with AQ in the presence of control IgG, IL-2 neutralizing antibody (BD Pharmingen, S4B6), or IL-2R blocking antibody (PC61 or 3 C7) for 2 days before harvest. (A) Cells were harvested at day 3 and incubated with anti-CD25 antibody (PE-PC61 or FITC-7D4) followed by flow cytometry analysis. A representative experiment of three independent experiments is shown. (B,C) Total RNA was prepared with TRIzol and subjected to reverse transcription and quantitative real time-PCR analysis. *p < 0.05 and ***p < 0.0005. Relative transcript levels of CD25 (B) and CD132 (C) were determined after normalization to the actin level from three independent experiments.

Back to article page