Figure 5 | Scientific Reports

Figure 5

From: Rab-GTPase binding effector protein 2 (RABEP2) is a primed substrate for Glycogen Synthase kinase-3 (GSK3)

Figure 5

Knockdown of GSK3β reduces endogenous RABEP2 production in H9C2 cells. H9C2 cells had shRNA constructs for each GSK3 isoform inserted using a lentiviral stable expression system (Sigma Mission particles). Clones with robust reduction in GSK3 isoform protein production were isolated and representative lines, along with a GFP expressing line generated at the same time, were treated with or without 10 μM CT99021 or DMSO carrier for 1 h. Cell lysates were probed with the antibodies as indicated.

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