Table 2 Sequences of peptides radiolabeled by GSK3.

From: Rab-GTPase binding effector protein 2 (RABEP2) is a primed substrate for Glycogen Synthase kinase-3 (GSK3)

HPLC Peak

m/z

M (Expt)

M (Theor.)

Mascot Score

Sequence (aa 196–212)

A

1031.4820

2060.9494

2060.9472

38

E.LLPLsRDPsPPLEPLEE.L + 2Phospho

B

991.4981

1980.9816

1980.9809

61

E.LLPLsRDPSPPLEPLEE.L + Phospho

B

1031.4818

2060.9490

2060.9472

48

E.LLPLsRDPsPPLEPLEE.L + 2Phospho

  1. The fractions from the HPLC column in Fig. 2A were analysed by in-line scintillation counting and radioactive fractions were subjected to Mass Fingerprinting for sequence detection. “s” in the Sequence indicates a Phosphorylated Serine residue. For the Mascot Score, an individual ion score of >21 indicates identity or extensive homology (p < 0.05). For the Mono-Phospho peptide; the Mascot delta score is >99.4% and the phosphoRS probability score is 100% respectively for S200 being PhosphoSerine. Peptide marked as * in Fig. 2A was unidentifiable.
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