Figure 2 | Scientific Reports

Figure 2

From: Development of a plasmid free CRISPR-Cas9 system for the genetic modification of Mucor circinelloides

Figure 2

Genome editing strategy designed to disrupt the carB gene of Mucor circinelloides by the CRISPR-Cas9 method and positions of the primers used in the study (A) and PCR analysis of the transformants using the MccarB1 and MccarB2 primers (Table 2) (B). TGG indicates the PAM sequence; arrows show the orientations of the primers. Panel B: M, GeneRuler 1 kb DNA ruler (Thermo Scientific); 1, MS12 strain; 2, MS12-carB + pyrG/1 strain; 3, MS12- carB + pyrG/2 strain.

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